In vitro innervation as an experimental model to study the expression and functions of acetylcholinesterase and agrin in human skeletal muscle [Elektronski vir]Miš, Katarina ...Acetylcholinesterase (AChE) and agrin, a heparan-sulfate proteoglycan, reside in the basal lamina of the neuromuscular junction (NMJ) and play key roles in cholinergic transmission and ... synaptogenesis. Unlike most NMJ components, AChE and agrin are expressed in skeletal muscle and [alpha]-motor neurons. AChE and agrin are also expressed in various other types of cells, where they have important alternative functions that are not related to their classical roles in NMJ. In this review, we first focus on co-cultures of embryonic rat spinal cord explants with human skeletal muscle cells as an experimental model to study functional innervation in vitro. We describe how this heterologous rat-human model, which enables experimentation on highly developed contracting human myotubes, offers unique opportunities for AChE and agrin research. We then highlight innovative approaches that were used to address salient questions regarding expression and alternative functions of AChE and agrin in developing human skeletal muscle. Results obtained in co-cultures are compared with those obtained in other models in the context of general advances in the field of AChE and agrin neurobiology.Source: Molecules [Elektronski vir]. - ISSN 1420-3049 (Vol. 22, iss. 9, 2017, str. 1-30)Type of material - e-articlePublish date - 2017Language - englishCOBISS.SI-ID - 33379545
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Select the library membership card:
DRS, in which the journal is indexed
Select pickup location:
Material pickup by post
Subject headings in COBISS General List of Subject Headings
Select pickup location
Please wait a moment.