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Raziskave topnosti in antioksidativne aktivnosti izvlečka vej bele jelke (Abies alba) = Research on the solubility and antioxidant activity of white fir branch extract (Abies alba)Udovič, Maja, farmacevtkaRaziskave izvlečka iz bele jelke so pripeljale do ugotovitve, da ima izvleček antiaterosklerotično, kardioprotektivno ter antipsoriastično delovanje in zmanjšuje post-prandialni glikemični odziv. ... Izvleček vsebuje ogljikove hidrate in polifenole, ki z znano biološko, farmakološko in klinično aktivnostjo prispevajo k delovanju. Naš namen je bil z metodama lovljenja DPPH in ABTS+ radikalov ugotoviti antioksidativno aktivnost v izvlečku iz vej bele jelke. Z modifikacijo metod, kjer za ugotavljanje antioksidativne aktivnosti namesto metanola uporabimo vodo, dobimo realnejšo sliko, kaj se po zaužitju izvlečka dogaja v našem telesu. Zanimivo je bilo spoznanje, da ob menjavi topila iz vode v metanol ali obratno pride do obarjanja določenih snovi, ki lahko vplivajo na antioksidativno aktivnost. Koncentracija reagentov vpliva na meritev antioksidativne aktivnosti. Najboljši parameter za ugotavljanje antioksidativnega delovanja z DPPH metodo je za standarda galno kislino in kvercetin naklon premice, za izvlečka Belinal in Belinal C pa IC50. Za metodo ABTS+ je za standarda in izvlečka naklon premice najboljši parameter. S centrifugiranjem in filtracijo smo ugotovili, da delež izvlečka, ki se raztopi, znaša od 37 % do 98 %, kar je odvisno od vrste vzorca, metode, topila ter od razmerja med topilom in vzorcem. Z metodo centrifugiranja smo ugotavljali, kolikšen delež vzorca je netopen v metanolu ali v vodi, netopen v obeh topilih ali topen v le enem topilu. Z metodo filtriranja smo preverjali topnost izvlečka v različnih razmerjih med izvlečkom in vodo ter 0,01 M HCl. Belinal je v obeh topilih bolje topen kot Belinal C. Na topnost in vsebnost antioksidantov v izvlečku lahko vpliva leto poseka, obdobje poseka vej, okolje, proces predelave vej in starost vzorca ob izvajanju testov, ker vsi ti dejavniki vplivajo na sestavo izvlečka.Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [M. Udovič], 2020Language - slovenianCOBISS.SI-ID - 34107651
Author
Udovič, Maja, farmacevtka
Other authors
Kreft, Samo
Topics
Topnost |
Antioksidanti |
antioksidanti |
bela jelka |
izvleček iz vej |
topila |
topnost
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Library/institution |
City | Acronym | For loan | Other holdings |
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Faculty of Pharmacy, Ljubljana | Ljubljana | FFALJ |
reading room 1 cop.
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Udovič, Maja, farmacevtka | ![]() |
Kreft, Samo | 15460 |
Source: Personal bibliographies
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