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Vpliv gliv na razkroj velikih lesnih ostankov bukve v pragozdnem rezervatu Rajhenavski Rog : diplomsko delo - univerzitetni študij - 1. stopnja = The role of fungi in the decay of coarse woody debris of European Beech (Fagus Sylvatica L.) in the forest reserve Rajhenavski Rog : B. Sc. Thesis - Academic study programmesFinžgar, DomenUgotavljanje vpliva gliv na razgradnjo velikih lesnih ostankov (VLO) v pragozdnem rezervatu smo razčlenili na dva dela. Prvi se je ukvarjal z ugotavljanjem zmanjševanja gostot VLO s stopnjo ... razgradnje, drugi pa z opisom pojavnosti gliv v vzorcih VLO z metodo gelske elektroforeze v gradientu denaturantov (DGGE). Z namenom ugotavljanja zanesljivosti drugačnih, zdravju varnejših metod od živosrebrne metode, tudi na vodnem volumometru. Pri tem je bila pilotno uporabljena tehtnica Sartorius MSA 323S z dodatkom za merjenje gostot YDK01. Merili smo osnovne, nasičene in absolutne gostote. Po pričakovanjih sta nasičeni in osnovni gostoti pokazali trend padanja gostot. Tehtnica Sartorius se je izkazala za zanesljivo in hitro metodo merjenja gostot v vodi, ki pa jo bo potrebno še optimizirati. Molekularne metode ugotavljanja prisotnosti gliv v VLO so v Sloveniji v razvoju. Na podlagi ekstrakcije glivne DNA iz vzorcev VLO smo s pomočjo analitske metode DGGE uspeli pridobiti profile združb gliv za posamezni vzorec. Rezultati so pokazali, da so nekatere glive značilne za začetne stopnje razkroja, druge pa za končne stopnje. Pestrost profilov se s stopnjo razkroja veča, odstopa le stopnja razkroja 1, ki ima nepričakovano veliko stopnjo pestrosti. Razvrstitev vzorcev glede na podobnost DGGE profilov ni bila skladna z razvrstitvijo v stopnje razgradnje, variabilnosti med vzorci znotraj stopenj razgradnje je bila visoka. Povezave med genetskimi in geografskimi razdaljami niso bile ugotovljene.Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [D. Finžgar], 2013Language - slovenianCOBISS.SI-ID - 3715750
Author
Finžgar, Domen
Other authors
Kraigher, Hojka
Topics
bukev |
DGGE |
gostota |
glive |
Rajhenav |
VLO |
voda |
živo srebro
Library/institution |
City | Acronym | For loan | Other holdings |
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Slovenian Forestry Institute, Forestry Library, Ljubljana | Ljubljana | GIS |
outside loan 2 cop.
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Shelf entry
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Finžgar, Domen | 37939 |
Kraigher, Hojka | 07127 |
Source: Personal bibliographies
and: SICRIS
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