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  • Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex [Elektronski vir]
    Minias, Alina ...
    Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as ... emerging human pathogens. MABC consists of 3 subspecies - M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing.
    Source: Scientific reports [Elektronski vir]. - ISSN 2045-2322 (Vol. 10, 2020, str. 1-9)
    Type of material - e-article ; adult, serious
    Publish date - 2020
    Language - english
    COBISS.SI-ID - 38347779

    Link(s):

    https://www.nature.com/articles/s41598-020-73607-x.pdf
    DiRROS - Digital repository of Slovenian research organizations
    DOI

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