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Characterization of artificially generated PbS aerosols and their use within a respiratory bioaccessibility test
Beeston, Michael Philip ...
A new method is presented for the preparation, characterization and use of PbS (galena) nanoparticles within an in vitro bioaccessibility test representing the respiratory tract, specifically the ...conditions occurring in conjunction with phagocytosis by cells using artificial lysosomal fluid. Particle production through nanosecond laser ablation enables their rapid production with a relatively narrow particle size distribution, and a diameterenabling them to represent particles that can enter the alveolar region of the respiratory tract (<3 [mu]m). The PbS nanoparticles were characterized by cascade impaction to define their particle size distribution and through the use of X-ray diffraction (XRD) and electron microprobe analysis (EMPA) to define their mineralogy and homogeneity respectively. The particles were collected via liquid impingement in artificial lysosomal fluid and the undissolved material was separated via ultrafiltration after a contact time of 7-140 hours to define the bioaccessibility.
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