-
Endocrine activities and adipogenic effects of bisphenol AF and its main metaboliteGramec, Darja, farmacevtka ...Bisphenol AF (BPAF) is a fluorinated analog of bisphenol A (BPA), and it is a more potent estrogen receptor (ER) agonist. BPAF is mainly metabolized to BPAF-glucuronide (BPAF-G), which has been ... reported to lack ER agonist activity and is believed to be biologically inactive. The main goal of the current study was to examine the influence of the metabolism of BPAF via glucuronidation on its ER activity and adipogenesis. Also, as metabolites can have different biological activities, the effects of BPAF-G on other nuclear receptors were evaluated. First, in-vitro BPAF glucuronidation was investigated using recombinant human enzymes. Specific reporter-gene assays were used to determine BPAF and BPAF-G effects on estrogen, androgen, glucocorticoid, and thyroid receptor pathways, and on PXR, FXR, and PPAR[gamma] pathways. Their effects on lipid accumulation and differentiation were determined in murine 3T3L1 preadipocytes using Nile Red, with mRNA expression analysis of the adipogenic markers adiponectin, Fabp4, Ceb[alpha], and PPAR[gamma]. BPAF showed strong agonistic activity for hER[alpha] and moderate antagonistic activities for androgen and thyroid receptors, and for PXR. BPAF-G was antagonistic for PXR and PPAR[gamma]. BPAF (0.1%microM) and BPAF-G (1.0%microM) induced lipid accumulation and increased expression of key adipogenic markers in murine preadipocytes. BPAF-G is therefore not an inactive metabolite of BPAF. Further toxicological and epidemiological investigations of BPAF effects on human health are warranted, to provide better understanding of the metabolic end-elimination of BPAF.Source: Chemosphere. - ISSN 0045-6535 (Vol. 215, Jan. 2019, str. 870-880)Type of material - article, component partPublish date - 2019Language - englishCOBISS.SI-ID - 4620401
Author
Gramec, Darja, farmacevtka |
Carino, Adriana |
Trontelj, Jurij |
Troberg, Johanna |
Distrutti, Eleonora |
Marchianò, Silvia |
Tomašič, Tihomir |
Zega, Anamarija |
Finel, Moshe |
Fiorucci, Stefano |
Peterlin-Mašič, Lucija
Topics
Farmacevtska kemija |
bisphenol AF |
bisphenol AF glucuronide |
glucuronidation endocrine activities |
lipid accumulation
Author | Gramec, Darja, farmacevtka ... |
Title | Endocrine activities and adipogenic effects of bisphenol AF and its main metabolite |
Publication date | 2019-01-29 |
COBISS.SI-ID | 4620401 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin | P1-0208-2015 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
Files that belong to the publication
Link |
---|
https://www.sciencedirect.com/science/article/pii/S0045653518319908#! |
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Gramec, Darja, farmacevtka | 35432 |
Carino, Adriana | |
Trontelj, Jurij | 23420 |
Troberg, Johanna | |
Distrutti, Eleonora | |
Marchianò, Silvia | |
Tomašič, Tihomir | 28334 |
Zega, Anamarija | 18633 |
Finel, Moshe | |
Fiorucci, Stefano | |
Peterlin-Mašič, Lucija | 19317 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.