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Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitorsVovk, Irena, 1965- ...The separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by ... conventional TLC silica gel 60plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates. For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. Plates were developed in modified horizontal developing chamber using ethyl acetate-acetone-acetic acid-water (4:1:0.25:0.5, v/v). Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and afterexposure to iodine also by image analysis. The obtained results showed that monolithic layer is more efficient for the separation of structurally similar polar compounds, such as prilates than conventional silica layers. Identification of the compounds was confirmed by ESI-MS after their on-line extraction from the UTLC and TLC plates by means of Camag TLC-MS interface.Source: Journal of chromatography. A. - ISSN 0021-9673 (Vol. 1218, no. 20, 2011, str. 3089-3094)Type of material - article, component partPublish date - 2011Language - englishCOBISS.SI-ID - 4647962
Author
Vovk, Irena, 1965- |
Popović, Gordana, kemičarka |
Simonovska, Breda |
Albreht, Alen |
Agbaba, Danica
Topics
analizna kemija |
spektrometrija |
kromatografija
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Vovk, Irena, 1965- | 11395 |
Popović, Gordana, kemičarka | ![]() |
Simonovska, Breda | 15461 |
Albreht, Alen | 29488 |
Agbaba, Danica | ![]() |
Source: Personal bibliographies
and: SICRIS
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