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Določanje trombocitnih protiteles s pretočno citometrijo = Determination of platelet antibodies by flow cytometry : magistrski študij laboratorijske biomedicine
Bizjak, Anja, biomedicina
Imunska trombocitopenija (ITP) je pridobljena avtoimunska bolezen, za katero je značilno zmanjšano število trombocitov (manj kot 100%109/L). Patogeneza bolezni še ni popolnoma pojasnjena, saj so pri ...razvoju bolezni vključeni različni mehanizmi. Namen naloge je bil določiti uporabnost pretočne citometrije pri postavitvi diagnoze ITP. Pri tem smo uporabljali komercialno dostopen komplet reagentov ThrombocytestTMimmune. 41 vzorce v smo analizirali na dveh pretočnih citometrih, določili občutljivost, specifičnost, pozitivno in negativno napovedno vrednost. Da bi lahko z večjo zagotovostjo ocenili uporabnost pretočne citometrije pri diagnozi ITP, bi potrebovali večje število vzorcev bolnikov z ITP, predvsem z akutnim ITP, kot kontrolno skupino vzorce samo popolnoma zdravih oseb in analizo trombocitnih protiteles skozi daljše časovno obobje. Kljub temu menimo, da lahko določitev trombocitnih protiteles s pretočno citometrijo pripomore pri postavitvi diagnoze ITP.
Type of material - master's thesis
; adult, serious
Publication and manufacture - Ljubljana : [A. Bizjak], 2019
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