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13.
Evaluation of the Amplicor HCV test: experiences after 1 year of routine use in a diagnostic laboratory
Seme, Katja, 1966- ; Poljak, Mario, 1965-
To evaluate the usefulness of a standardized commercial amplication assay for detection of hepatitis C virus (HCV) RNA, amplicor HCV test (Rhoche), a total of 1,204 serum samples from 888 patients ...was examined. Seven out of 443 anti-HCV-negative samples, 638 out of 729 anti-HCV-positive samples and four out 32 anti-HCV-indeterminate samples tested HCV RNA positive by amplicor in initial testing. One false-negative and five false-positive amplicor result were found in initial testing, giving an overall sensitivity and specificity of the amplicor HCV test 99,8% and 99.1%, respectively. Our study confirmed that the amplicor HCV test is a practical, rapid, sensitive and specific assay for detection of HCV RNA. However, the results must be interpreted with some caution and with the patient's anti-HCV status and clinical data in mind.
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