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Munc18-1 and Munc18-2 proteins modulate ß-cell Ca2+ sensitivity and kinetics of insulin exocytosis differentlyMandic, SA ...Fast neurotransmission and slower hormone release share the same core fusion machinery consisting of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins. In evoked ... neurotransmission, interactions between SNAREs and the Munc18-1 protein, a member of the Sec1/Munc18 (SM) protein family, are essential for exocytosis, whereas other SM proteins are dispensable. To address if the exclusivity of Munc18-1 demonstrated in neuroexocytosis also applied to fast insulin secretion, we characterized the presence and function of Munc18-1 and its closest homologue Munc18-2 in ß-cell stimulus-secretion coupling. We show that pancreatic ß-cells express both Munc18-1 and Munc18-2. The two Munc18 homologues exhibit different subcellular localization, and only Munc18-1 redistributes in response to glucose stimulation. However, both Munc18-1 and Munc18-2 augment glucose-stimulated hormone release. Ramp-like photorelease of caged Ca2+ and high resolution whole-cell patch clamp recordings show that Munc18-1 and Munc18-2 overexpression shift the Ca2+ sensitivity of the fastest phase of insulin exocytosis differently. In addition, we reveal that Ca2+ sensitivity of exocytosis in ß-cells depends on the phosphorylation status of the Munc18 proteins. Even though Munc18-1 emerges as the key SM-protein determining the Ca2+ threshold for triggering secretory activity in a stimulated ß-cell, Munc18-2 has the ability to increase Ca2+ sensitivity and thus mediates the release of fusion-competent granules requiring a lower cytoplasmic-free Ca2+ concentration, [Ca2+]i. Hence, Munc18-1 and Munc18-2 display distinct subcellular compartmentalization and can coordinate the insulin exocytotic process differently as a consequence of the actual [Ca2+]i.Source: The Journal of biological chemistry. - ISSN 0021-9258 (Vol. 286, no. 32, 2011, str. 28026-28040)Type of material - article, component part ; adult, seriousPublish date - 2011Language - englishCOBISS.SI-ID - 512148536
Author
Mandic, SA |
Skelin, Maša |
Johansson, JU |
Rupnik, Marjan |
Berggren, Per-Olof |
Bark, C.
Topics
Munc18 Proteins |
physiology
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source: The Journal of biological chemistry. - ISSN 0021-9258 (Vol. 286, no. 32, 2011, str. 28026-28040)
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Mandic, SA | ![]() |
Skelin, Maša | 29565 |
Johansson, JU | ![]() |
Rupnik, Marjan | 12266 |
Berggren, Per-Olof | ![]() |
Bark, C. | ![]() |
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