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  • Glucose-stimulated calcium dynamics in Islets of Langerhans in acute mouse pancreas tissue slices [Elektronski vir]
    Stožer, Andraž ; Dolenšek, Jurij ; Rupnik, Marjan
    In endocrine cells within islets of Langerhans calcium ions couple cell stimulation to hormone secretion. Since the advent of modern fluorimetry, numerous in vitro studies employing primarily ... isolated mouse islets have investigated the effects of various secretagogues on cytoplasmic calcium, predominantly in insulin-secreting beta cells. Due to technical limitations, insights of these studies are inherently limited to a rather small subpopulation of outermost cells. The results also seem to depend on various factors, like culture conditions and duration, and are not always easily reconcilable with findings in vivo. The main controversies regard the types ofcalcium oscillations, presence of calcium waves, and the level of synchronized activity. Here, we set out to combine the in situ acute mouse pancreas tissue slice preparation with noninvasive fluorescent calcium labeling and subsequent confocal laser scanning microscopy to shed new light on the existing controversies utilizing an innovative approach enabling the characterization of responses in many cells from all layers of islets. Our experiments reproducibly showed stable fast calcium oscillations on a sustained plateau rather than slow oscillations as the predominant type of response in acute tissue slices, and that calcium waves are the mechanistic substrate for synchronization of oscillations. We also found indirect evidencethat even a large amplitude calcium signal was not sufficient and thatmetabolic activation was necessary to ensure cell synchronization upon stimulation with glucose. Our novel method helped resolve existing controversies and showed the potential to help answer important physiological questions, making it one of the methods of choice for the foreseeable future.
    Source: PloS one [Elektronski vir]. - ISSN 1932-6203 (Vol. 8, iss. 1, 2013, str. 1-13)
    Type of material - e-article ; adult, serious
    Publish date - 2013
    Language - english
    COBISS.SI-ID - 512254008

    Link(s):

    http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0054638
    Digital Library of the University of Maribor – DLUM
    DOI

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