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  • Functional connectivity in islets of Langerhans from mouse pancreas tissue slices [Elektronski vir]
    Stožer, Andraž ...
    We propose a network representation of electrically coupled beta cells in islets of Langerhans. Beta cells are functionally connected on the basis of correlations between calcium dynamics of ... individual cells, obtained by means of confocal laser-scanning calcium imaging in islets from acute mouse pancreastissue slices. Obtained functional networks are analyzed in the light of known structural and physiological properties of islets. Focusing on the temporal evolution of the network under stimulation with glucose, we show thatthe dynamics are more correlated under stimulation than under non-stimulated conditions and that the highest overall correlation, largely independent of Euclidean distances between cells, is observed in the activation and deactivation phases when cells are driven by the external stimulus. Moreover, we find that the range of interactions in networks during activity shows a clear dependence on the Euclidean distance, lending support to previous observations that beta cells are synchronized via calcium waves spreading throughout islets. Most interestingly, the functional connectivity patterns between beta cells exhibit small-world properties, suggesting that beta cells do not form a homogeneous geometric network but are connected in a functionally more efficient way. Presented results provide support for the existing knowledge of beta cell physiology from a network perspective and shedimportant new light on the functional organization of beta cell syncitia whose structural topology is probably not as trivial as believed so far.
    Source: PLoS computational biology. - ISSN 1553-734X (Vol. 9, iss. 2, Feb. 2013, str. e100292312-1-e1002923-12)
    Type of material - e-article ; adult, serious
    Publish date - 2013
    Language - english
    COBISS.SI-ID - 512264760

    Link(s):

    Digital Library of the University of Maribor – DLUM
    DOI

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