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Potentiation of electrochemotherapy effectiveness by immunostimulation with IL-12 gene electrotransfer in mice is dependent on tumor immune statusUršič Valentinuzzi, Katja, 1990- ...Electrochemotherapy (ECT) exhibits high therapeutic effectiveness in the clinic, achieving up to 80% local tumor control but without a systemic (abscopal) effect. Therefore, we designed a combination ... therapy consisting of ECT via intratumoral application of bleomycin, oxaliplatin or cisplatin with peritumoral gene electrotransfer of a plasmid encoding interleukin-12 (p. t. IL-12 GET). Our hypothesis was that p. t. IL-12 GET potentiates the effect of ECT on local and systemic levels and that the potentiation varies depending on tumor immune status. Therefore, the combination therapy was tested in three immunologically different murine tumor models. In poorly immunogenic B16F10 melanoma, IL-12 potentiated the antitumor effect of ECT with biologically equivalent low doses of cisplatin, oxaliplatin or bleomycin. The most pronounced potentiation was observed after ECT using cisplatin, resulting in a complete response rate of 38% and an abscopal effect. Compared to B16F10 melanoma, better responsiveness to ECT was observed in more immunogenic 4%T1 mammary carcinoma and CT26 colorectal carcinoma. In both models, p. t. IL-12 GET did not significantly improve the therapeutic outcome of ECT using any of the chemotherapeutic drugs. Collectively, the effectiveness of the combination therapy depends on tumor immune status. ECT was more effective in more immunogenic tumors, but GET exhibited greater contribution in less immunogenic tumors. Thus, the selection of the therapy, namely, either ECT alone or combination therapy with p. t. IL-12, should be predominantly based on tumor immune status.Source: Journal of controlled release. - ISSN 0168-3659 (Vol. 332, no. 21, Apr. 2021, str. 623-635)Type of material - article, component partPublish date - 2021Language - englishCOBISS.SI-ID - 54492931
Author
Uršič Valentinuzzi, Katja, 1990- |
Kos, Špela, farmacija |
Kamenšek, Urška |
Čemažar, Maja |
Miceska, Simona |
Markelc, Boštjan |
Buček, Simon, 1983- |
Lisec, Barbara, 1991- |
Kloboves-Prevodnik, Veronika |
Heller, Richard |
Serša, Gregor
Topics
elektrokemoterapija |
elektroprenos genov |
interlevkin-12 |
electrochemotherapy |
gene electrotransfer |
interleukin-12
Author | Uršič Valentinuzzi, Katja, 1990- ... |
Title | Potentiation of electrochemotherapy effectiveness by immunostimulation with IL-12 gene electrotransfer in mice is dependent on tumor immune status |
Publication date | 2021-03-08 |
COBISS.SI-ID | 54492931 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Spremljanje imunskega odziva v realnem času po in situ vakcinaciji z elektrokemoterapijo in pridruženo gensko terapijo z interlevkinom-12 | J3-8202-2017 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Značilnosti malignih neoplazem, pomembne za diagnozo ter napoved poteka bolezni in izida zdravljenja | P3-0289-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Razvoj in ovrednotenje novih terapij za zdravljenje malignih tumorjev | P3-0003-2015 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Elektrokemoterapija kot in situ vakcinacija | Z3-2651-2020 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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4815.80 € : Onkološki inštitut Ljubljana |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Uršič Valentinuzzi, Katja, 1990- | 38223 |
Kos, Špela, farmacija | 36366 |
Kamenšek, Urška | 28387 |
Čemažar, Maja | 14575 |
Miceska, Simona | 51443 |
Markelc, Boštjan | 32175 |
Buček, Simon, 1983- | 39955 |
Lisec, Barbara, 1991- | 39122 |
Kloboves-Prevodnik, Veronika | 15076 |
Heller, Richard | |
Serša, Gregor | 08800 |
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