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Evaluation of a novel plasmid for simultaneous gene electrotransfer-mediated silencing of CD105 and CD146 in combination with irradiation [Elektronski vir]Savarin, Monika ...Targeting tumor vasculature through specific endothelial cell markers represents a promising approach for cancer treatment. Here our aim was to construct an antibiotic resistance gene-free plasmid ... encoding shRNAs to simultaneously target two endothelial cell markers, CD105 and CD146, and to test its functionality and therapeutic potential in vitro when delivered by gene electrotransfer (GET) and combined with irradiation (IR). Functionality of the plasmid was evaluated by determining the silencing of the targeted genes using qRT-PCR. Antiproliferative and antiangiogenic effects were determined by the cytotoxicity assay tube formation assay and wound healing assay in murine endothelial cells 2H-11. The functionality of the plasmid construct was also evaluated in malignant melanoma tumor cell line B16F10. Additionally, potential activation of immune response was measured by induction of DNA sensor STING and proinflammatory cytokines by qRT-PCR in endothelial cells 2H-11. We demonstrated that the plasmid construction was successful and can efficiently silence the expression of the two targeted genes. As a consequence of silencing, reduced migration rate and angiogenic potential was confirmed in 2H-11 endothelial cells. Furthermore, induction of DNA sensor STING and proinflammatory cytokines were determined, which could add to the therapeutic effectiveness when used in vivo. To conclude, we successfully constructed a novel plasmid DNA with two shRNAs, which holds a great promise for further in vivo testing.Source: International journal of molecular sciences [Elektronski vir]. - ISSN 1422-0067 (Vol. 22, 2021, str. 3069-1-3069-18)Type of material - e-articlePublish date - 2021Language - englishCOBISS.SI-ID - 56427011
Author
Savarin, Monika |
Kamenšek, Urška |
Žnidar, Katarina |
Todorović, Vesna, 1982- |
Serša, Gregor |
Čemažar, Maja
Topics
CD105 |
CD146 |
plazmidi |
elektroprenos genov |
CD105 |
CD146 |
plasmid |
gene electrotransfer
Author | Savarin, Monika ... |
Title | Evaluation of a novel plasmid for simultaneous gene electrotransfer-mediated silencing of CD105 and CD146 in combination with irradiation [Elektronski vir] |
Publication date | 2021-03-17 |
COBISS.SI-ID | 56427011 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Nov pristop žilno ciljane terapije, kjer z gensko terapijo utišamo dve neodvisni signalni poti, v kombinaciji z radioterapijo | Z3-1873-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Razvoj in ovrednotenje novih terapij za zdravljenje malignih tumorjev | P3-0003-2015 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
Files that belong to the publication
Link |
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https://www.mdpi.com/journal/ijms |
https://dirros.openscience.si/IzpisGradiva.php?id=15568 |
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Savarin, Monika | 35355 |
Kamenšek, Urška | 28387 |
Žnidar, Katarina | 37534 |
Todorović, Vesna, 1982- | 29469 |
Serša, Gregor | 08800 |
Čemažar, Maja | 14575 |
Source: Personal bibliographies
and: SICRIS
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