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On-cartridge preparation and evaluation of 68Ga-, 89Zr- and 64Cu-precursors for cell radiolabellingSočan, Aljaž ...Introduction Indium-111 when formulated as indium-111 oxine remains the gold standard for long term cell tracking, whereas radiometals for improved PET applications still have to be established. We ... here describe the on-cartridge formation of gallium-68, zirconium-89 and copper-64 complexes in small volumes suitable for cell labelling, including labelling of red blood cells (RBC) and white blood cells (WBC) and their biological evaluation in vivo. Methods Small volumes (1%2%mL) of tracers (oxine, tropolone) were directly prepared on an anion exchange cartridge (Sep-Pak QMA). Cells were radiolabelled and the labelling efficiency and efflux were evaluated. The in vivo biodistribution of copper-64-labelled WBC using [64Cu][Cu(oxinate)2] and [64Cu][Cu(tropolonate)2] was monitored in an infection and inflammation animal model using BALB/c mice. Results On-cartridge concentration of gallium-68, zirconium-89 and copper-64 enabled formation of oxine and tropolone tracers in small volumes with good yields (%50%) and quality (extraction %90%). Prepared tracers radiolabelled the RBC comparable to indium-111 tracers and in vivo biodistribution of copper-64 labelled WBC showed clear accumulation of cells at the site of infection and inflammation. Conclusions This on-cartridge preparation method enables simple formation of various PET tracers for cell radiolabelling. Zirconium-89 and copper-64 tracers radiolabelled cells with sufficient stability. Due to their longer half-life this approach could be promising for routine applications where longer evaluation periods for cell tracking are needed. Advances in knowledge and implications for patient care This novel approach for on-cartridge concentration and preparation of oxine and tropolone precursors with different positron emitters, in small volume and suitable pH, offers a versatile tool towards cell labelling for preclinical and clinical PET applications.Source: Nuclear medicine and biology. - ISSN 0969-8051 (Vol. 71, 2019, str. 23-31)Type of material - article, component partPublish date - 2019Language - englishCOBISS.SI-ID - 6031276
Author
Sočan, Aljaž |
Petrik, Miloš |
Kolenc, Petra, 1976- |
Krošelj, Marko |
Rangger, Christine |
Nový, Zbyšek |
Švajger, Urban |
Gmeiner, Tanja |
Decristoforo, Clemens
Topics
cell labelling |
PET |
Gallium-68 |
Copper-64 |
Zirconium-89 |
on-cartridge complex formation
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Sočan, Aljaž | 29238 |
Petrik, Miloš | |
Kolenc, Petra, 1976- | 23364 |
Krošelj, Marko | 31672 |
Rangger, Christine | |
Nový, Zbyšek | |
Švajger, Urban | 26198 |
Gmeiner, Tanja | 15706 |
Decristoforo, Clemens |
Source: Personal bibliographies
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