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Detection of anti HIV-1/2 antibodies in saliva of HIV-seropositive persons in Slovenia = Prisotnost protiteles proti HIV-1/2 v slini oseb, okuženih s HIV, v SlovenijiMatičič, Mojca ...Background. To evaluate the sensitivity and specificitity of saliva-baed anti HIV antibody testing in the Slovenian population due to heterogeneity of HIV. Patients and methods. Serum and saliva ... samples from 35 persons previously determined as HIV-1 seropositive, and 50 persons of unknown HIV status with norisk factors for HIV infection were evaluated in a prospective, blinded fashion for anti HIV-1/2 antibodies. The clinical classificantion of HIV infection, serum HIV-1 RNA viral load and antiretroviral drug use were wel documented in all HIV-seropositive persons. Saliva was gathered with the Omni-Sal collection device designed to concentrate crevicular fluid rich saliva, and analysed for the presence of anti HIV-1/2 antibodies using the commercial enzyme immuno assay Wellocozyme HIV 1+2 GACELISA, and confirmatory Western blot test. Independently, serum samples were analysed for anti HIV-1/2antibodies using two different commercial screening enzyme immuno assays (Wellcozyme HIV 1+2, Enzygnost Anti-HIV 1+2), and three different confirmatory Western blot tests. Results. All 35 persons previously determinedas HIV-1 seropositive, expressing most various stages of HIV diseasewith a wide range of serum HIV-1 RNA load, and different variations in antiretroviral therapy, had detectable antiodies in saliva by both screening and confirmatory test. None of the 50 controls were positive by testing of serum or saliva specimens for the presence of anti HIV-1/2 antibodies. According to our study both the sensitivity and specificity of Wellcozyme HIV 1+2 Gacelisa were 100%. Conclusions. Our study confirmed that testing of saliva appears to be a simple, safe, sensitive and specific method for detecting anti HIV antibodies, used mostly for surveillance purposes.Source: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 67, suppl. 2, June 1998, str. II-83 - II-86)Type of material - article, component partPublish date - 1998Language - englishCOBISS.SI-ID - 7618265
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source: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 67, suppl. 2, June 1998, str. II-83 - II-86)
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Matičič, Mojca | 11348 |
Poljak, Mario, 1965- | 11622 |
Tomažič, Janez, 1954- | 11347 |
Vidmar, Ludvik | 12180 |
Artnik, Barbara | 20687 |
Skalerič, Uroš, 1945-2023 | 03370 |
Source: Personal bibliographies
and: SICRIS
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