The suitability of a glovebox and of a covered still air box design for semi-sterile applications in environmental monitoring
Kosel, Janez ; Ropret, Polonca, kemik
Laminar flow cabinets (LFCs) ensure a safe working space within which product manipulation can be carried out safely excluding contaminations of the product with the environmental microorganisms. ...However, for environmental monitoring applications mobile laboratories are required and these prefer the lighter gloveboxes (GB; restricted arm movement) or still air boxes (SAB; free arm movement) over the heavier, more expensive LFCs, which need to be regularly maintained. Nevertheless, the efficiency of simple GBs/SABs (no HEPA filter), in providing semi-sterile working conditions has yet to be clearly defined. Consequently, our aim was to assess the suitability of GBs/SABs for semi-sterile applications by using passive and active bioaerosol sample collection procedures within the interior spaces of these boxes. Prior to sample collection the boxes were pre-treated with different spraying preparations (70% ethanol, 2% detergent or sterile water). For a greater restriction of bioaerosol entry, SABs were constructed with covered arm ports and these were classified as partially covered (SABPC) and completely covered SABs (SABCC). Results showed that ethanol sprayed GB and SABCC exhibited microbial aerosol colony counts of zero after one hour of passive sample collection, and active sample collection revealed counts ranging between 1.9 (for GB) - 2.3 Log10CFU/m3 (for SABCC). However, ethanol sprayed SAB and SABPC were ineffective having colony counts of 6.9 and 6.5 Log10CFU/m3, respectively. Other spraying regimes resulted in even higher colony counts (up to 7.3 Log10CFU/m3). Therefore, the ethanol sprayed GB and SABCC could effectively be used for semi-sterile applications, with the SABCC allowing for an unrestricted arm movement within it.
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
PDF
