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Robust saliva-based RNA extraction-free one-step nucleic acid amplification test for mass SARS-CoV-2 monitoring [Elektronski vir]Rajh, Eva, biokemičarka ...Early diagnosis with rapid detection of the virus plays a key role in preventing the spread of infection and in treating patients effectively. In order to address the need for a straightforward ... detection of SARS-CoV-2 infection and assessment of viral spread, we developed rapid, sensitive, extraction-free one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP) tests for detecting SARS-CoV-2 in saliva. We analyzed over 700 matched pairs of saliva and nasopharyngeal swab (NSB) specimens from asymptomatic and symptomatic individuals. Saliva, as either an oral cavity swab or passive drool, was collected in an RNA stabilization buffer. The stabilized saliva specimens were heat-treated and directly analyzed without RNA extraction. The diagnostic sensitivity of saliva-based RT-qPCR was at least 95% in individuals with subclinical infection and outperformed RT-LAMP, which had at least 70% sensitivity when compared to NSBs analyzed with a clinical RT-qPCR test. The diagnostic sensitivity for passive drool saliva was higher than that of oral cavity swab specimens (95% and 87%, respectively). A rapid, sensitive one-step extraction-free RT-qPCR test for detecting SARS-CoV-2 in passive drool saliva is operationally simple and can be easily implemented using existing testing sites, thus allowing high-throughput, rapid, and repeated testing of large populations. Furthermore, saliva testing is adequate to detect individuals in an asymptomatic screening program and can help improve voluntary screening compliance for those individuals averse to various forms of nasal collections.Source: Molecules [Elektronski vir]. - ISSN 1420-3049 (Vol. 26, iss. 21, art. 6617, 2021, str. 1-19)Type of material - e-article ; adult, seriousPublish date - 2021Language - englishCOBISS.SI-ID - 83744003
Author
Rajh, Eva, biokemičarka |
Šket, Tina, mikrobiologija |
Praznik, Arne |
Sušjan, Petra |
Šmid, Alenka, farmacevtka |
Urbančič, Dunja, farmacevtka |
Mlinarič-Raščan, Irena |
Kogovšek, Polona, 1978- |
Demšar, Tina, 1973- |
Milavec, Mojca, 1972- |
Prosenc, Katarina |
Jensterle, Žiga |
Zidarn, Mihaela |
Tomič, Viktorija |
Turel, Gabriele |
Lejko-Zupanc, Tatjana |
Jerala, Roman |
Benčina, Mojca, 1968-
Topics
SARS-CoV-2 |
COVID-19 serological testing |
Real-time polymerase chain reaction |
Saliva |
Covid-19 |
SARS-CoV-2 |
Serološko testiranje za COVID-19 |
Verižna reakcija s polimerazo v realnem času |
Slina |
Covid-19 |
bris ustne sluznice |
združeni vzorci |
oral cavity swab |
passive drool |
pooling
Author | Rajh, Eva, biokemičarka ... |
Title | Robust saliva-based RNA extraction-free one-step nucleic acid amplification test for mass SARS-CoV-2 monitoring [Elektronski vir] |
Publication date | 2021-10-29 |
COBISS.SI-ID | 83744003 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Okoljska in aplikativna virologija: virusi, prijatelji in sovražniki | P4-0407-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Biotehnologija in sistemska biologija rastlin | P4-0165-2015 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin | P1-0208-2015 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Molekularna biotehnologija: od dinamike bioloških sistemov do aplikacij | P4-0176-2015 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
DNK cepiva in peptidnih inhibitorji proti SARS-CoV-2 | V4-2038-2020 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
Files that belong to the publication
Link |
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https://www.mdpi.com/1420-3049/26/21/6617/pdf |
https://dirros.openscience.si/IzpisGradiva.php?id=14590 |
https://repozitorij.uni-lj.si/IzpisGradiva.php?id=136425 |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Rajh, Eva, biokemičarka | 55264 |
Šket, Tina, mikrobiologija | 54583 |
Praznik, Arne | 50616 |
Sušjan, Petra | 37644 |
Šmid, Alenka, farmacevtka | 29982 |
Urbančič, Dunja, farmacevtka | 34512 |
Mlinarič-Raščan, Irena | 12443 |
Kogovšek, Polona, 1978- | 25523 |
Demšar, Tina, 1973- | 19994 |
Milavec, Mojca, 1972- | 19119 |
Prosenc, Katarina | 21847 |
Jensterle, Žiga | |
Zidarn, Mihaela | 25317 |
Tomič, Viktorija | 22806 |
Turel, Gabriele | 50050 |
Lejko-Zupanc, Tatjana | 11330 |
Jerala, Roman | 06628 |
Benčina, Mojca, 1968- | 14360 |
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