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  • Adhesion and stiffness of detached breast cancer cells in vitro [Elektronski vir] : co-treatment with metformin and 2-deoxy-d-glucose induces changes related to increased metastatic potential
    Zemljič Jokhadar, Špela ...
    Metastatic cancer cells can overcome detachment-induced cell death and can proliferate in anchorage-independent conditions. A recent study revealed that a co-treatment with two drugs that interfere ... with cell metabolism, metformin and 2-deoxy-D-glucose, promotes detachment of viable MDA-MB-231 breast cancer cells. In the present study, we analyzed if these detached viable MDA-MB-231 cells also exhibit other features related to cancer metastatic potential, i.e., if they are softer and more prone to adhere to epithelial cells. The cell mechanics of attached cells and floating cells were analyzed by optical tweezers and cell deformability cytometry, respectively. The adhesion was assessed on a confluent monolayer of HUVEC cells, with MDA-MB-231 cells either in static conditions or in a microfluidic flow. Additionally, to test if adhesion was affected by the state of the epithelial glycocalyx, HUVEC cells were treated with neuraminidase and tunicamycin. It was found that the treated MDA-MB-231 cells were more prone to adhere to HUVEC cells and that they were softer than the control, both in the floating state and after re-seeding to a substrate. The changes in the HUVEC glycocalyx, however, did not increase the adhesion potential of MDA-MB-231.
    Source: Biology [Elektronski vir]. - ISSN 2079-7737 (Vol. 10, iss. 9, 2021, str. 1-15)
    Type of material - e-article ; adult, serious
    Publish date - 2021
    Language - english
    COBISS.SI-ID - 87303939

    Link(s):

    https://www.mdpi.com/2079-7737/10/9/873
    Repository of the University of Ljubljana – RUL
    DOI

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