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The cytotoxic and cytolytic activity of equinatoxin II from the sea anemone Actinia equinaBatista, Urška ; Maček, Peter ; Sedmak, Bojan, 1952-The cytotoxic and cytolytic effects of equinatoxin II (EqT II) from the sea anemone Actinia equina L. were studied on exponentially growing and synchronized V-79-379 A cell line in culture. The cell ... viability test and the determination of the cytolytic effect by cell confirmed both cytotoxic and cytolytic activity of EqT II. Additionally, cytocidal and cytostatic effects depending on the toxin concentration were observed. The presence of fetal calf serum in the cell culture medium reduced both cytocidal and cytostatic effects by two magnitudes and prevented cytolysis. Combining EqT II and serum resulted in an insoluble complex which was cytostatic even when isolated and resuspended in the culture medium, while the supernatant retained both cytocidal and cytostatic activity. No significant difference insensitivity between synchronized and exponentially growing cells could be detected after EqT II treatment.Source: Cell biology international reports. - ISSN 0309-1651 (Vol. 14, No. 11, 1990, str. 1013-1024)Type of material - article, component part ; adult, seriousPublish date - 1990Language - englishCOBISS.SI-ID - 901967
Author
Batista, Urška |
Maček, Peter |
Sedmak, Bojan, 1952-
Topics
biochemistry |
equinatoxin II |
Actinia equina
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source: Cell biology international reports. - ISSN 0309-1651 (Vol. 14, No. 11, 1990, str. 1013-1024)
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Batista, Urška | 08588 |
Maček, Peter | 06994 |
Sedmak, Bojan, 1952- | 07736 |
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