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Izolacija mutirane različice proteina ostreolizina A6, OlyA6 E69A, in njena interakcija z umetnimi lipidnimi membranami : magistrsko delo : magistrski študij - 2. stopnja = Isolation of a mutated version of the ostreolysin A6 protein, OlyA6 E69A, and its interaction with artificial lipid membranes : M. Sc. Thesis : Master Study ProgrammesCerjak, NastjaProtein OlyA6 uvrščamo med egerolizine iz glivnega rodu ostrigarjev (Pleurotus), za katere je značilno, da se vežejo na nevretenčarski sfingolipid ceramid fosfoetanolamin (CPE). OlyA6 in njegova ... mutanta OlyA6 E69A se razlikujeta v eni sami aminoksilinski substituciji, kjer je glutamat na poziciji 69 zamenjan z alaninom. OlyA6 se veže na že prej formirane lipidne komplekse SM/Hol v membranah, OlyA6 E69A pa se lahko zaradi točkovne mutacije veže na lipidne membrane, ki vsebujejo SM, ki ni v kompleksu s Hol. V sklopu magistrske naloge smo protein OlyA6 E69A izolirali ter analizirali njegovo interakcijo z lipidnimi vezikli, ki vsebujejo CPE z ali brez dodanega holesterola. Prav tako smo preverili litičnost kompleksa OlyA6 E69A v prisotnosti proteinskega partnerja pleurotolizina B (PlyB) na lipidnih veziklih. Ugotovili smo, da se proteinski kompleks OlyA6 E69A/PlyB v primerjavi z OlyA6/PlyB bolje veže na lipidne vezikle, da za vezavo ne potrebuje Hol ter da jih učinkoviteje permeabilizira. Na vezavo in permeabilizacijo OlyA6 E69A vpliva tudi količina CPE ali SM, saj je vezava mutante boljša v primerih, ko je v membranah koncentracija teh lipidov višja.Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [N. Cerjak], 2022Language - slovenianCOBISS.SI-ID - 97120259
Author
Cerjak, Nastja
Other authors
Sepčić, Kristina |
Panevska, Anastasija |
Skočaj, Matej |
Turk, Tom, 1959-
Topics
ceramid fosfoetanolamin |
lipidna membrana |
ostreolizin A6 |
OlyA6 E69A |
sfingomielin |
ceramide phosphoethanolamine |
lipid membrane |
ostreolysin A6 |
OlyA6 E69A |
sphingomyelin
Library/institution |
City | Acronym | For loan | Other holdings |
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National Institute of Biology and BF, Department of Biology, Ljubljana | Ljubljana | BFBNIB |
reading room 1 cop.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Cerjak, Nastja | |
Sepčić, Kristina | 15328 |
Panevska, Anastasija | 39090 |
Skočaj, Matej | 33137 |
Turk, Tom, 1959- | 06905 |
Source: Personal bibliographies
and: SICRIS
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