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A correlation of NK cytolitic test and BLT esterase test in determining activity of NK cells, stimulated by tumor target cells = Korelacija med NK citolitičnim in BLT esteraznim testom pri določevanju aktivnosti NK celic stimuliranih s tumorskimi celicami
Ihan, Alojz
We examined the granule exocytosis in natural killer (NK) cells by measuring N-benzyloxycarboxy-L-lysine esterase activity. As a source of NK cells we usedbuffy-coat isolated NK cells or peripheral ...blood lymphocytes (PBL). The exocytosis was triggered by incubating cells with ionomycin/PMA or by NK cell susceptible tumour target cells K562. When we stimulated purified NK cells with tumour target cells, a close correlation (Corr=0.84) of cytolitic NK testresults and BLT test results was obtained. We may conclude that BLT test can provide a valuable tool to discriminate further NK cell deficiency in patients with low cytolitic NK test results.
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