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Filtracija in diafiltracija sirotke - optimizacija masnega izkoristka za laktoferin in laktoperoksidazo [Elektronski vir] = Whey filtration and diafiltration – optimization of mass yield for lactoferrin and lactoperoxidaseKete, Marko ...Pred kromatografsko izolacijo laktoferina (LF) in laktoperoksidaze (LPO) na kromatografski koloni je zaradi zaščite same kolone in njenega stabilnega delovanja potrebno sirotko prefiltrirati. Za ... izvedbo izolacij na pilotnem nivoju smo zato redno izvajali tudi filtracijo sirotke na pilotnem filtracijskem sistemu (JIUWU HI-TECH) in spremljali masno prehodnost sirotkinih proteinov (LF, LPO, IgG, aLA in bLG). Izkazalo se je, da na retentatni strani v večji meri zastajajo LF, LPO in IgG, medtem ko manjši proteini (aLA in bLG) lažje prehajajo membrano. To vodi do znižane koncentracije LF in LPO v permeatu po opravljeni filtraciji sirotke, kar se kaže v nižjem masnem izkoristku za LF in LPO. Z namenom dviga izkoristka filtracije smo opravili več primerjalnih filtracij in diafiltracij kisle in sladke sirotke, pri čemer smo vrednotili vpliv koncentracijskega faktorja ter v primeru diafiltracije, vpliv dodatka prečiščene vode, ki jo med filtracijo dodajamo na retentano stran, na masni izkoristek. Kot glavni zaključek lahko izpostavimo, da med masnimi izkoristki filtracije ali diafiltracije ni bilo bistvenih razlik. Izkazalo se je, da ima na izkoristek nekoliko večji vpliv koncentracijski faktor filtracije, pri čemer se z njegovim večanjem dviga tudi sam masni izkoristek postopka.Type of material - conference contribution ; adult, seriousPublish date - 2022Language - slovenian, englishCOBISS.SI-ID - 99377155
Author
Kete, Marko |
Osel, Nika, farmacevtka |
Oberčkal, Jernej |
Frančeškin Krapež, Mateja
Topics
filtracija |
diafiltracija |
sirotka |
masni izkoristek |
laktoferin |
laktoperoksidaza
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Kete, Marko | 31282 |
Osel, Nika, farmacevtka | 53673 |
Oberčkal, Jernej | 33324 |
Frančeškin Krapež, Mateja | 53197 |
Source: Personal bibliographies
and: SICRIS
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