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Iskanje antimutagenih učinkovin v polsteni mlečnici (Lactarius vellereus) = Searching for antimutagenic compounds in fleecy milkcap (Lactarius vellereus) : diplomska nalogaHorvat, Marija, farmacevtkaV okviru diplomskega dela smo iskali potencialno antimutagene učinkovine v glivi polsteni mlečnici (Lactarius vellereus). Količina in vrsta izoliranih spojin iz glive je odvisna od načina ... ekstrakcije, zato smo suhe izvlečke pripravili v različnih topilih in ob razliènih časih dodatka topila po poskodbi glive. Kot topila smo uporabili metanol, metanol z dodatkom kisline, etilacetat in heksan, topilo pa smo dodali takoj po poskodbi, pet ur in 24 ur po poskodbi. Iz vseh vzorcev smo pripravili suhe izvleèke. S SOS umu testom smo spremljali inhibicijo z UV zarki induciranega SOS odziva pri bakteriji Salmonella typhimurium TA 1535pSK1002 in izbrali najbolj optimalen izvleček za kasnejse delo. Ugotovili smo, da je od pripravljenih izvlečkov SOS odziv najbolj inhibiral metanolni izvleček, pri katerem smo glivi dodali metanol 24 ur po poskodbi. Pripravili smo večjo količino suhega izvlečka v metanolu, s časom dodatka topila po 24. urah in ga ločili na sestih kolonah, napolnjenih s silikagelom. Uporabili smo različne mobilne faze za kolone, stacionarne faze z različno velikostjo delcev silikagela in koloni dveh dimenzij. Zbrane frakcije s kolone smo ločili s tankoplastno kromatografijo in jih na podlagi podobnosti lis zdruzili, pripravili suhe izvlečke in jih preizkusili s SOS umu testom. Od vseh vzorcev, ki smo jih preizkusili, sta dva delovala zelotoksično na bakterije, saj sta zmanjsala prezivelost bakterij na okoli 10%. Od 60. vzorcev, ki smo jih dobili z zdruzevanjem frakcij iz kolone, jih je 12 inhibiralo SOS odziv (inhibicijski faktor pod 0.7).Frakcijo iz kolone, ki je inhibirala z UV zarki induciran SOS odziv (inhibicijski faktor 0.658), smo ločili se s pomočjo preparativne HPLC metode na 12 frakcij in jih preizkusili, vendar nobena ni izkazala močne inhibicije SOS odziva.Type of material - undergraduate thesisPublication and manufacture - Ljubljana : [Horvat M.], 2003Language - slovenianCOBISS.SI-ID - 1258865
Author
Horvat, Marija, farmacevtka
Other authors
Umek, Andrej, 1951- |
Filipič, Metka, 1954-
Topics
glive |
antimutageneza |
mutageneza |
Lactarius vellereus |
polstena mlečnica |
bakterijski test
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Database name | Field | Year |
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Horvat, Marija, farmacevtka | ![]() |
Umek, Andrej, 1951- | 09635 |
Filipič, Metka, 1954- | 09892 |
Source: Personal bibliographies
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