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Proučevanje litičnih bakteriofagov mlečno-kislinskih bakterij : magistrsko delo = Study of lactic acid bacteria lytic bacteriophages : master of science thesisMiklič, AndrejaTo je prva raziskava, ki proučuje bakteriofage mlečno-kislinskih bakterij, izoliranih iz slovenskih mlekarskih obratov. V obdobju dveh let smo izolirali trinajst laktokoknih fagov iz vzorcev sirotke ... treh mlekarskih obratov. Fagom smo določili morfološke lastnosti, gostiteljski obseg, restrikcijski profil, velikost gemoma in homolognost DNK. Vsi fagi pripadajo družini Siphoviridae. Štirje fagi imajo elipsoidno glavo (52-62 x 40-58 nm) in 88 do 95 nm dolg, nekrčljiv rep (morfotip B2,vrsta c2). Devet fagov ima majhno izometrično glavo (premer 46 do 63 nm) in dolg, nekrčljiv rep (morfotip B1). Fagi z elipsoidno glavo kažejo širši gostiteljski obseg kot fagi z majhno izometrično glavo. Na osnovi restrikcijskih fragmentov DNK in velikosti genoma lahko uvrstimo štiri fage (øKR7, øKR8, øKR36 in øKR43) v isto skupino (c2). Ocenjena velikost genomov elipsoidnih fagov je med 26,1 in 29,4 kb. Ti fagi kažejo močno homolognost DNK s tipskim fagom c5A. Velikost genoma fagov z majhno izometrično glavo je med 26,6 in 60,9 kb. Na osnovi homolognosti DNK, vsi izometrični fagi kažejo sorodnost s fagno vrsto 936.Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [A. Miklič-Anderlič], 1998Language - slovenianCOBISS.SI-ID - 2062968
Author
Miklič, Andreja
Other authors
Rogelj, Irena, 1955- |
Drinovec, Borut
Topics
virusi |
bakteriofagi |
mlečno-kislinske bakterije |
starter kulture |
izdelava sirov |
sirotka |
odpornost |
brstna velikost |
latentna doba |
razvrščanje fagov |
fagne vrste |
tipski fagi |
morfološke lastnosti |
genomska velikost |
homolognost DNK |
viruses |
bacteriophages |
lactic acid bacteria |
starters |
cheese making |
whey |
resistence |
burst size |
latent period |
phage classification |
phage species |
phage types |
morphological properties |
genome size |
DNA homology
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Miklič, Andreja | 15141 |
Rogelj, Irena, 1955- | 08857 |
Drinovec, Borut | 03174 |
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