BF, Dept. of Food Science and Technology, Lj. (BFZIV)
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13.
Proučevanje litičnih bakteriofagov mlečno-kislinskih bakterij : magistrsko delo = Study of lactic acid bacteria lytic bacteriophages : master of science thesis
Miklič, Andreja
To je prva raziskava, ki proučuje bakteriofage mlečno-kislinskih bakterij, izoliranih iz slovenskih mlekarskih obratov. V obdobju dveh let smo izolirali trinajst laktokoknih fagov iz vzorcev sirotke ...treh mlekarskih obratov. Fagom smo določili morfološke lastnosti, gostiteljski obseg, restrikcijski profil, velikost gemoma in homolognost DNK. Vsi fagi pripadajo družini Siphoviridae. Štirje fagi imajo elipsoidno glavo (52-62 x 40-58 nm) in 88 do 95 nm dolg, nekrčljiv rep (morfotip B2,vrsta c2). Devet fagov ima majhno izometrično glavo (premer 46 do 63 nm) in dolg, nekrčljiv rep (morfotip B1). Fagi z elipsoidno glavo kažejo širši gostiteljski obseg kot fagi z majhno izometrično glavo. Na osnovi restrikcijskih fragmentov DNK in velikosti genoma lahko uvrstimo štiri fage (øKR7, øKR8, øKR36 in øKR43) v isto skupino (c2). Ocenjena velikost genomov elipsoidnih fagov je med 26,1 in 29,4 kb. Ti fagi kažejo močno homolognost DNK s tipskim fagom c5A. Velikost genoma fagov z majhno izometrično glavo je med 26,6 in 60,9 kb. Na osnovi homolognosti DNK, vsi izometrični fagi kažejo sorodnost s fagno vrsto 936.
Type of material - master's thesis
; adult, serious
Publication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [A. Miklič-Anderlič], 1998
Knjižnica MD 0000000077 a IN: 0010810
Knjižnica MD 77 a IN: 0010810
available - outside loan, loan period: 1 months
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