Vrednotenje celične linije PC-12 s pretočno citometrijo s sočasnim zajemanjem slike = Evaluating of PC-12 cell line using imaging flow cytometry : univerzitetni študijski program Kozmetologija
Lipovnik, Anja
V diplomskem delu smo vrednotili celično linijo PC-12, ki predstavlja dober nevrološki model za raziskavo nevrodegenerativnih bolezni. Analize smo opravili s pretočno citometrijo s sočasnim ...zajemanjem slike. Slikovni citometer ImageStream združuje kovencionalno pretočno citometrijo in sodobno fluorescenčno mikroskopijo. Omogoča multiparametrične analize, s katerimi lahko hitro in učinkovito karakteriziramo lastnosti posameznih celic, kot so velikost, oblika, tekstura, granuliranost in intenzivnost fluorescence. Celice smo označili s fluorofornimi označevalci (DAPI, Alexa Fluor 488, Alexa Fluor 633, TRITC) neposredno, s faloidinom ali posredno s protitelesi. S pomočjo programske opreme IDEAS preučili delež živih celic PC-12 ter distribucijo aktina, cistatina C in katepsina X. S statistično analizo (določitev vrednost RD) smo na podlagi fenotipskih značilnosti celic ovrednotili subpopulacije in posamezne celice. Intenziteto fluorescence smo določili pri permeabiliziranih in nepermeabiliziranih celicah. Višje vrednosti intenzitete signala smo zaznali pri permeabiliziranih celicah, saj označena protitelesa lahko vstopijo v celice le, če je njihova membrana permeabilizirana.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [A. Lipovnik], 2019
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