Molekulski mehanizmi uravnavanja tvorbe maščobnega tkiva pri prašiču (Sus scrofa) : doktorska disertacija = Molecular background of fat tissue formation in pig (Sus Scrofa) : doctoral dissertation
Miloševič Berlič, Tamara
Izolirali smo kandidatne gene PGC-1, TFAM in UCP-1 prašiča, ki so vključeni v uravnavanje metabolizma maščobnega tkiva, in jih karakterizirali. Z RT-PCR in z RACE PCR smo pomnožili celotno kodirajočo ...regijo PGC-1 in TFAM ter pripadajoči 5'- in 3'-neprevedeni regiji obeh genov. S sekvenciranjem osmega eksona PGC-1 pri osmih različnih pasmah prašičev smo ugotovili tri genetske variante, ki smo jih lahko identificirali z analizo SSCP. S florescentno hibridizacijo in situ smo ugotovili kromosomsko lokalizacijo gena PGC-1 na osmem kromosomu prašiča (SSC8, p2.1-2.3). S sistemom tranlacije in vitro v pšeničnem ekstraktu smo pripravili rekombinantni protein TFAM in ga uporabili v testu EMSA ter za slednje z DNazo I. S sekvenciranjem potencialnih veznih mest za TFAM v D-loop regiji mtDNA različnih pasem prašičev smo ugotovili, da je ta del mtDNA polimorfen. Genotipizacija fragmentov gena UCP-1 v gelih MetaPhor je potrdila prisotnost dolžinskega polimorfizma v 3'-neprevedeni regiji tega gena. Ugotovili smo tudi nukleotidno zaporedje celotnega introna 3 in delno nukleotidno zaporedje eksonov 3 in 4 gena UCP-1 prašiča. Naši rezultati pomenijo prvo karakterizacijo genov PGC-1 in TFAM pri prašiču in odpirajo možnost za nadaljnje preučevanje vloge teh regulatornih proteinov v procesih adaptivne termogeneze in adipogeneze.
Type of material - dissertation
; adult, serious
Publication and manufacture - Ljubljana : [BF, Interdisciplinarni podiplomski študij biotehnologije] : [T. Miloševič Berlič], 2002
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
