Izolacija sevov Frankia sp. iz nodulov jelše (Alnus sp.)
Grebenc, Tine ...
Frankia je filamentoyna bakterija, ki fiksira duǴik in lahko tvori koreninske gomoljřke na veř kot dvesto vrstah dvokaliřnic. Predvsem je pomembna kot simbiont drevesnih vrst. Gojenje rodu Frankia v ...laboratorijskih raymerah je yahtevno in iyolacija sevov iy nodulov je teÍavna. V naǴi rayiskavi smo preiykusili rayliřne metode iyolacije. Iyhodni material so bili koreninski gomoljřki pri dveh vrstah jelǴe, Alnus glutinosa in A. incana, nabrani na rayliřnih lokacijah in v rayliřnih obdobjih leta. Macerate povrǴinsko steriliyiranih gomoljřkov smo po filtraciji ali centrifugiranju v ultracentrifugi nacepili v rayliřna gojiǴřa, vendar tudi po dolgotrajni inkubaciji nikjer nismo opayili rasti. Pař pa smo y macerati uspeǴno inokulirali sadike jelǴe v laboratorijskih raymerah (v rastnih Íepih). Pri tako dobljenih sevih rodu Frankia smo nato ugotavljali sposobnost fiksacije duǴika. Prav tako smo nodule uporabili ya ekstrakcijo DNK in molekularno karakteriyacijo rayliřnih iyolatov na osnovi pomnoÍevanja (PCR) ribosomalnih genov in genov ya fiksacijo duǴika.
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