Nekužna kultura seva A5969 Mycoplasma gallisepticum sintetizira rekombinanten adhezin pMGA = Non infectious culture of Mycoplasma gallisepticum strain A5969 synthesizes a recombinant pMGA adhezin
Dovč, Peter, 1958- ...
Mycoplasma gallisepticum sodi med najpomembnejše povzročitelje respiratornih bolezni perutnine. Njena membrana vsebuje številne adhezine in med njimi je najbolje izražen lipoprotein pMGA, ki ga ...kodira velika družina genov. Ugotovili smo, da sevi M. gellisepticum lahko prenehajo izražati značilno 67 kDa obliko pMGA in namesto nje izražajo protein pMGA, ki se razlikuje po velikosti (40-80kDa) in izraženih epitopih. Antigenske variante pMGA, ki izražajo različne epitope, nastanejo kot posledica reverzibilnih točkovnih sprememb v genih pMGA. Sev A5969 RE je HA pozitiven (HA+). Takšen fenotip je povezan z antigenskimi različicami velikosti 48, 50 in 80 kDa in ne z 69 kDa obliko pMGA. Nekužna kultura seva A5969 SHK M. gallisepticum (>500 pasaž) sintetizira 69 kDa pMGA, medtem ko nizka pasaža seva A5969 GE izraža značilno obliko pMGA. Kultura A5969 SHK ima podaljšan N-terminalni del molekule pMGA in zaporedje aminokislin, ki je homologno pMGA1.9 Protein pMGA (69 kDa), ki ga izraža kultura A5969 SHK je rekombinanten (preklopi s pMGA1.9 v 1.2 v 1.1 v 1.2).
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