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  • Microtiter plate assay for phosphate using a euporium-tetracyline complex as a sensitive luminiscent probe
    Dürkop, Axel ...
    A new luminescent europium probe is presented for the determination of phosphate (P) in microtiter plate format. The assay is based on the quenching of the luminescence of the europium-tetracycline ... (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 ▫$\mu$▫ mol/L. The probe is excited at 400 nm and displays a large Stokes' shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7 and is therefore suitable for phosphate determination in biological and biochemical systems. The linear range of the calibration plot is from 5 x ▫$10^{-6}▫ mol/L to 7.5 x ▫$10{-4}$▫ mol/L of phosphate, and the limit of detection is 3 ▫$\mu$▫ mol/L.
    Vir: Analytica chimica acta. - ISSN 0003-2670 (Vol. 555, iss. 2, 2006, str. 292-298)
    Vrsta gradiva - članek, sestavni del
    Leto - 2006
    Jezik - angleški
    COBISS.SI-ID - 10105110

    Povezava(-e):

    http://dx.doi.org/10.1016/j.aca.2005.09.007

vir: Analytica chimica acta. - ISSN 0003-2670 (Vol. 555, iss. 2, 2006, str. 292-298)
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