-
The activation of GPR27 increases cytosolic L-lactate in 3T3 embryonic cells and astrocytes [Elektronski vir]Dolanc, Dorian ...G-protein-coupled receptors (GPCRs) represent a family with over 800 members in humans, and one-third of these are targets for approved drugs. A large number of GPCRs have unknown physiologic roles. ... Here, we investigated GPR27, an orphan GPCR belonging to the family of super conserved receptor expressed in the brain, with unknown functions. Cytosolic levels of L-lactate ([lactate]i), the end product of aerobic glycolysis, were measured with the Laconic fluorescence resonance energy transfer nanosensor. In single 3T3 wild-type (WT) embryonic cells, the application of 8535 (1 µM), a surrogate agonist known to activate GPR27, resulted in an increase in [lactate]i. Similarly, an increase was recorded in primary rat astrocytes, a type of neuroglial cell abundant in the brain, which contain glycogen and express enzymes of aerobic glycolysis. In CRISPR-Cas9 GPR27 knocked out 3T3 cells, the 8535-induced increase in [lactate]i was reduced compared with WT controls. Transfection of the GPR27-carrying plasmid into the 3T3KOGPR27 cells rescued the 8535-induced increase in [lactate]i. These results indicate that stimulation of GPR27 enhances aerobic glycolysis and L-lactate production in 3T3 cells and astrocytes. Interestingly, in the absence of GPR27 in 3T3 cells, resting [lactate]i was increased in comparison with controls, further supporting the view that GPR27 regulates L-lactate homeostasis.Vir: Cells [Elektronski vir]. - ISSN 2073-4409 (Vol. 11, iss. 6, 2022, str. 1-14)Vrsta gradiva - e-članek ; neleposlovje za odrasleLeto - 2022Jezik - angleškiCOBISS.SI-ID - 101228291
Avtor
Dolanc, Dorian |
Zorec, Tomaž Mark |
Smole, Zala |
Maver, Anja, biokemičarka |
Horvat, Anemari |
Pillaiyar, Thanigaimalai |
Trkov, Saša |
Vardjan, Nina |
Kreft, Marko |
Chowdhury Haque, Helena |
Zorec, Robert, 1958-
Teme
3T3 embrionalne celice |
astrociti |
aerobna glikoliza |
3T3 embryonic cells |
astrocytes |
aerobic glycolysis
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Dolanc, Dorian | |
Zorec, Tomaž Mark | 38244 |
Smole, Zala | 53467 |
Maver, Anja, biokemičarka | |
Horvat, Anemari | 36369 |
Pillaiyar, Thanigaimalai | |
Trkov, Saša | 31572 |
Vardjan, Nina | 20214 |
Kreft, Marko | 15666 |
Chowdhury Haque, Helena | 18548 |
Zorec, Robert, 1958- | 03702 |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.