VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Systems view of Bacillus subtilis pellicle development [Elektronski vir]
    Krajnc, Mojca, biotehnologinja ...
    In this study, we link pellicle development at the water–air interface with the vertical distribution and viability of the individual B. subtilis PS-216 cells throughout the water column. Real-time ... interfacial rheology and time-lapse confocal laser scanning microscopy were combined to correlate mechanical properties with morphological changes (aggregation status, filament formation, pellicle thickness, spore formation) of the growing pellicle. Six key events were identified in B. subtilis pellicle formation that are accompanied by a major change in viscoelastic and morphology behaviour of the pellicle. The results imply that pellicle development is a multifaceted response to a changing environment induced by bacterial growth that causes population redistribution within the model system, reduction of the viable habitat to the water–air interface, cell development, and morphogenesis. The outcome is a build-up of mechanical stress supporting structure that eventually, due to nutrient deprivation, reaches the finite thickness. After prolonged incubation, the formed pellicle collapses, which correlates with the spore releasing process. The pellicle loses the ability to support mechanical stress, which marks the end of the pellicle life cycle and entry of the system into the dormant state.
    Vir: npj biofilms and microbiomes [Elektronski vir]. - ISSN 2055-5008 (Vol. 8, 2022, str. 1-11, art. 25)
    Vrsta gradiva - e-članek ; neleposlovje za odrasle
    Leto - 2022
    Jezik - angleški
    COBISS.SI-ID - 105098243

    Povezava(-e):

    https://www.nature.com/articles/s41522-022-00293-0
    Repozitorij Univerze v Ljubljani – RUL
    DOI

Vnos na polico