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  • Determining the parameters of concentration-response relationship for histamine on the isolated portion of guinea pig stomach and guinea pig right atrium
    Stanovnik, Lovro
    In our previous experiments measuring acid secretion in the isolated mouse stomach we found higher EC50 values (agonist concentiation evoking half maximal effect for histamine than in the experiments ... on the isolated guinea pig right atrium (contraction frequency). The difference could be due to nonequilibrium conditions in the isolated mouse stomach preparation or to species differences. In order to check this possibility and to test the consistency of concentration-response relationship (CRR) parameter determination on an isolated secretory organ, we compared EC50 for histamine and pA2 for famotidine on the two isolated preparations from the same species,guinea pigs. Both, EC50 and pA2 values were significantly different when measuring gastric acid secretion (9.7 micro M and 6.07) from the parameters, found in the isolated right atrium measuring frequency of contractions (1.6 micro M and 7.06). From these results it can be concluded that the isolated portion of guinea pig stomach is suitable for CRR determination; but the parameters obtained are in the range of higher concentrations than the heal ones because of kinetic particularities of the preparation.
    Vir: Book of abstracts and programme (str. R197-R199)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2000
    Jezik - angleški
    COBISS.SI-ID - 12195801

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