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  • ATR-FTIR spectroscopy as a pre-screening technique for the PMI assessment and DNA preservation in human skeletal remains – a review
    Leskovar, Tamara ...
    Skeletal remains from archaeological and forensic contexts are commonly used for various analyses, including DNA. Our research investigates relatively fast and inexpensive ATR-FTIR spectroscopy with ... further data manipulation as a potential pre-screening technique for assessing the preservation state of skeletal remains. We seek to correlate spectra and chemometric indices with the biological, chemical, and physical composition of the skeletal tissue, and the quality and quantity of DNA preserved in the tissue, also considering the skeletal element, absolute age, and environmental conditions to which the remains have been exposed. Our research focuses on human bones and teeth derived from archaeological, WWII and forensic contexts. The samples are cleaned and pulverized according to established methodological procedures for DNA extraction. DNA is extracted and quantified, while the remaining powder is analysed by ATR-FTIR spectroscopy. The obtained spectra are manipulated to extract the chemometric indices, which are the base for statistical analyses, machine learning, and prediction algorithms. A compilation of results from several studies shows that ATR-FTIR spectra present a solid base for researchers to better understand the preservation state of phosphates, carbonates, and collagen in the skeletal tissues, assessment of post-mortem interval, and the preservation of the DNA. Although many questions still need to be answered, ATR-FTIR spectroscopy with further data manipulation has a high potential for research of taphonomic changes, PMI and DNA preservation in human skeletal remains.
    Vir: Quaternary international. - ISSN 1040-6182 (Vol. 660, June 2023, str. 56-64)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2023
    Jezik - angleški
    COBISS.SI-ID - 123458819

    Povezava(-e):

    https://www.sciencedirect.com/science/article/pii/S1040618222003044
    Repozitorij Univerze v Ljubljani – RUL
    DOI

vir: Quaternary international. - ISSN 1040-6182 (Vol. 660, June 2023, str. 56-64)
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