VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
PDF
  • Surface modified magnetic nanoparticles for immuno-gene therapy of murine mammary adenocarcinoma
    Prijič, Sara ...
    Cancer immuno-gene therapy is an introduction of nucleic acids encoding immunostimulatory proteins, such as cytokine interleukin 12 (IL-12), into somatic cells to stimulate an immune response against ... a tumor. Various methods can be used for the introduction of nucleic acids into cells; magnetofection involves binding of nucleic acids to magnetic nanoparticles with subsequent exposure to an external magnetic field. Here we show that surface modified superparamagnetic iron oxide nanoparticles (SPIONs) with a combination of polyacrylic acid (PAA) and polyethylenimine (PEI) (SPIONs-PAA-PEI) proved to be safe and effective for magnetofection of cells and tumors in mice. Magnetofection of cells with plasmid DNA encoding reportergene using SPIONs-PAA-PEI was superior in transfection efficiency to commercially available SPIONs. Magnetofection of murine mammary adenocarcinoma with plasmid DNA encoding IL-12 using SPIONs-PAA-PEI resulted insignificant antitumor effect and could be further refined for cancer immuno-gene therapy.
    Vir: Biomaterials. - ISSN 0142-9612 (Vol. 33, iss. 17, 2012, str. 4379-4391)
    Vrsta gradiva - članek, sestavni del
    Leto - 2012
    Jezik - angleški
    COBISS.SI-ID - 1237883
    DOI

vir: Biomaterials. - ISSN 0142-9612 (Vol. 33, iss. 17, 2012, str. 4379-4391)
loading ...
loading ...
loading ...

Vnos na polico