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Housekeeping gene gyrA, a potential molecular marker for Bacillus ecology study [Elektronski vir]Liu, Yan, mikrobiolog ...Bacillus is a genus of microorganisms (bacteria) and contains many important commercial species used in industry, agriculture and healthcare. Many different Bacilli are relatively well understood at ... the single-cell level; however, molecular tools that determine the diversity and ecology of Bacillus community are limited, which limits our understanding of how the Bacillus community works. In the present study, we investigated the potential of the housekeeping gene gyrA as a molecular marker for determining the diversity of Bacillus species. The amplification efficiency for Bacillus species diversity could be greatly improved by primer design. Therefore, we designed a novel primer pair gyrA3 that can detect at least 92 Bacillus species and related species. For B. amyloliquefaciens, B. pumilus, and B. megaterium, we observed that the high variability of the gyrA gene allows for more detailed clustering at the subspecies level that cannot be achieved by the 16S rRNA gene. Since gyrA provides better phylogenetic resolution than 16S rRNA and informs on the diversity of the Bacillus community, we propose that the gyrA gene may have broad application prospects in the study of Bacillus ecology.Vir: AMB express [Elektronski vir]. - ISSN 2191-0855 (Vol. 12, 2022, str. 1-12)Vrsta gradiva - e-članek ; neleposlovje za odrasleLeto - 2022Jezik - angleškiCOBISS.SI-ID - 127325187
Avtor
Liu, Yan, mikrobiolog |
Štefanič, Polonca, mikrobna ekologinja |
Miao, Youzhi |
Xue, Yansheng |
Xun, Weibing |
Zhang, Nan |
Shen, Qirong |
Zhang, Ruifu |
Xu, Zhihui |
Mandić-Mulec, Ines
Teme
mikrobne združbe |
Bacillus |
ekologija mikroorganizmov |
molekularni markerji |
molecular marker |
16S rRNA |
Housekeeping gyrA gene |
SNPs |
Bacillus diversity
Vnos na polico
Trajna povezava
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Liu, Yan, mikrobiolog | |
Štefanič, Polonca, mikrobna ekologinja | 26540 |
Miao, Youzhi | |
Xue, Yansheng | |
Xun, Weibing | |
Zhang, Nan | |
Shen, Qirong | |
Zhang, Ruifu | |
Xu, Zhihui | |
Mandić-Mulec, Ines | 05993 |
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