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  • Searching for the physiological function of 17beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus: studies of substrate specificity and expression analysis
    Lanišnik-Rižner, Tea ; Stojan, Jure, 1956- ; Adamski, Jerzy, molekularna biologija
    17beta-hydroxysteroid dehydrogenase from the filamentous fungus Cochliobolus lunatus (17beta-HSDcI) has recently been characterized. Since its function is still unclear, we performed substrate ... specificity studies to obtain some indications about its physiological function. Different steroids were studied as putative substrates of recombinant 17beta-HSDcl, androgens and estrogens, brassinosteroids, and the fungal steroid herbarulid. Among these androgens andestrogens were most efficiently converted. The following substrates in decreasing order were best reduced: 4-estrene-3,17-dione, 5alpha-androstane-3,17-dione, 4-androstene-3,17-dione and estrone. Two typicalinhibitors were tested: carbenoxolone - a representative inhibitor of the SDR family and quercetin - a diagnostic inhibitor of carbonyl reductases. Among these two quercetin was more efficient. Expression studies revealed that17beta-HSDcl is mainly expressed in the stationary phase of growth indicating its possible involvement in secondary metabolism.
    Vir: Molecular and cellular endocrinology. - ISSN 0303-7207 (Letn. 171, 2001, str. 193-198)
    Vrsta gradiva - članek, sestavni del
    Leto - 2001
    Jezik - angleški
    COBISS.SI-ID - 12914905