VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Differentiation of glial cells and motor neurons during the formation of neuromuscular junctions in co-cultures of rat spinal cord explant and human muscle
    Marš, Tomaž, 1972- ...
    Motor axons extending from embryonic rat spinal cord explants form fully mature neuromuscular junctions with cocultured human muscle. This degree of maturation is not observed in muscle innervated by ... dissociated motor neurons. Glial cells present in the spinal cord explants seem to be, besides remaining interneurons, the major difference between the two culture systems. In light of this observation and the well documented role of glia in neuronal development, it can be hypothesized that differentiated and long-lived neuromuscular junctions form in vitro only if their formation is accompanied by codifferentiation of neuronal and glial cells and if this codifferentiationfollows the spatial and temporal pattern observed in vivo. Investigation of this hypothesis necessitates the characterization of neuronaland glial cell development in spinal cord explant-muscle cocultures. No such study has been reported, although these cocultures have been used in numerous studies of neuromuscular junction formation. The aim of this work wastherefore to investigate the temporal relationship between neuromuscular junction formation and the differentiation of neuronal and glial cells during the first 3 weeks of coculture, when formation and development of the neuromuscular junction occurs in vitro. The expression of stage-specific markers of neuronal and glial differentiation in these cocultures was characterized by immunocytochemical and biochemical analyses. Differentiation of astrocytes, Schwann cells, and oligodendrocytes proceeded in concert with the differentiation of motor neurons and neuromuscular junction formation. Thetemporal coincidence between maturation of the neuromuscular junction andlineage progression of neurons and glial cells was similar to that observedin vivo.(Abstract truncated at 2000 characters)
    Vir: Journal of comparative neurology. - ISSN 0021-9967 (Letn. 438, št. 2, 2001, str. 239-251)
    Vrsta gradiva - članek, sestavni del
    Leto - 2001
    Jezik - angleški
    COBISS.SI-ID - 13844441

vir: Journal of comparative neurology. - ISSN 0021-9967 (Letn. 438, št. 2, 2001, str. 239-251)
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