-
A novel approach of direct ex vivo epitope mapping identifies dominant and subdominant CD4 and CD8 T cell epitopes from Listeria monocytogenesGeginat, Gernot ...We used a novel approach for the direct ex vivo identification and characterization of T cell epitopes based on the screening of peptide spot libraries with freshly isolated splenocytes in a ... sensitive enzyme-linked immunospot (ELISPOT) assay. This technique was applied for the analysis of splenocytes from Listeria monocytogenes-infected BALB/c and C57BL/6 mice. The screening of peptide spot libraries covering the whole listeriolysin O and p60of L. monocytogenes confirmed all known CD4 and CD8 T cell epitopes of these proteins and additionally revealed six new H-2(d) and six new H-2(b)-restricted T cell epitopes. New epitopes were categorized into CD4 and CD8 T cell epitopes by ex vivo ELISPOT analysis with separated T cell populations.The quantitative analysis of cells reactive with these CD4 and CD8T cell epitopes revealed the existence of dominant and subdominant CD4 and CD8 T cell populations during L. monocytogenes infection. As a consequence of these data we suggest that ELISPOT-based screening of peptide spot libraries could be a general approach for the rapid identification and characterization of pathogen-specific T cell populations during various infectious diseases.Vir: The journal of immunology. - ISSN 0022-1767 (Letn. 166, št. 3, 2001, str. 1877-1884)Vrsta gradiva - članek, sestavni delLeto - 2001Jezik - angleškiCOBISS.SI-ID - 14371801
Avtor
Geginat, Gernot |
Schenk, Simone |
Škoberne, Mojca, 1972- |
Goebel, Werner |
Hof, Herbert
Teme
Listeria monocytogenes |
Immunology |
Epitope mapping |
Epitopes, t-lymphocyte |
Analysis |
Immunology |
Cd4-positive t-lymphocytes |
Immunology |
Microbiology |
Metabolism |
Cd8-positive t-lymphocytes |
Immunology |
Microbiology |
Metabolism |
Mice, inbred balb c |
Mice, inbred c57bl |
T-lymphocyte subsets |
Peptide library |
Lymphocyte transformation |
Immunophenotyping |
Immunologic memory |
Histocompatibility antigens class i |
Histocompatibility antigens class ii |
Heat-shock proteins |
Cell line |
Enzyme-linked immunosorbent assay |
Bacterial proteins |
Immunology |
Analysis |
Epitop, mapiranje |
Epitopi |
Limfociti T |
Listeria monocytogenes |
Bakterijske beljakovine |
Celična linija |
ELISA |
Histokompatibilnost, antigeni |
Imunofenotipizacija |
Imunski spomin |
Limfocit, transformacija |
Limfocitne podenote |
Miši seva BALB C |
Miši seva C57BL |
Peptidna knjižnica |
Vročinski-šok beljakovine
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Škoberne, Mojca, 1972- | 18356 |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.