-
Anti-platelet factor 4/heparin antibodies from patients with heparin-induced thrombocytopenia provoke direct activation of microvascular endothelial cellsBlank, Miri ...Heparin-induced thrombocytopenia (HIT) is a serious complication that occurs in -1-5% of patients treated with heparin and may be associated with severe thrombotic events. HIT is mediated by ... antibodies directed mostly to epitope(s)formed by complexes between heparin or other anionic mucopolysaccharides and platelet factor 4 (PF4). Anti-PF4/heparin IgG antibodies from six patients with HIT were affinity purified and assessed for interaction with human microvascular and macrovascular endothelial cells (EC).The antibodies directly activated primary cultures of human bone marrow microvascular EC (HBMEC) and SV40 immortalized HBMEC (TrHBMEC) only in the presence of PF4, but did not activate macrovascular human umbilical vein EC (HUVEC) under the same conditions. These antibodies were found to bind to TrHBMEC through the F(ab)2 portion of the anti-PF4/heparin IgG. TrHBMEC activation was characterized by an augmented release of IL-6, von Willebrand factor, soluble thrombomodulin, and by an elevated expression of the adhesion molecules P-selectin, E-selectin and vascular cellular endothelial molecule-I to different degrees. Enhanced monocyte adhesion to PF4/heparin antibody-treated TrHBMEC (33-72% adhesion) was also observed. None of these effects occurred with unstimulated HUVEC. However, pre-treatment of HUVEC withtumor necrosis factor-a resulted in the same changes observed with microvascular EC exposed to the HIT antibodies. Our findings indicate that anti-PF4/heparin antibodies directly activate microvascular EC while interaction with macrovascular EC requires pre-activation. These results may explain some of the specific clinical manifestations in HIT.Vir: International immunology. - ISSN 0953-8178 (Letn. 14, št. 2, 2002, str. 121-129)Vrsta gradiva - članek, sestavni delLeto - 2002Jezik - angleškiCOBISS.SI-ID - 14753497
Avtor
Blank, Miri |
Shoenfeld, Yehuda |
Tavor, Sigal |
Praprotnik, Sonja |
Boffa, Marie-Claire |
Weksler, Babette |
Walenga, Jeanine M |
Amiral, Jean |
Eldor, Amiram
Teme
Thrombocytopenia |
Chemically induced |
Heparin |
Adverse effects |
Platelet factor 4 |
IgG |
Epitopes |
Endothelium, vascular |
Antibodies, anti-idiotypic |
Interleukin-6 |
Von willebrand factor |
P-selectin |
E-selectin |
Vascular cell adhesion molecule-1 |
Bone marrow |
Cells, cultured |
Heparin |
IgG |
Trombocitni faktor 4 |
Trombocitopenija |
Celične kulture |
E-selektin |
Endotelij žilni |
Epitopi |
Interlevkin-6 |
Kostni mozeg |
P-selektin |
Protitelesa protiidiopatska |
Vaskularnocelična adhezijska molekula-1 |
von Willebrand faktor
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Praprotnik, Sonja | 13602 |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.