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  • Endoglin (CD105) silencing mediated by shRNA under the control of Endothelin-1 promoter for targeted gene therapy of Melanoma [Elektronski vir]
    Tešić, Nataša ...
    Endoglin (CD105), a transforming growth factor (TGF)-% coreceptor, and endothelin-1, a vasoconstrictor peptide, are both overexpressed in tumor endothelial and melanoma cells. Their targeting is ... therefore a promising therapeutic approach for melanoma tumors. The aim of our study was to construct a eukaryotic expression plasmid encoding the shRNA molecules against CD105 under the control of endothelin-1 promoter and to evaluate its therapeutic potential both in vitro in murine B16F10-luc melanoma and SVEC4-10 endothelial cells and in vivo in mice bearing highly metastatic B16F10-luc tumors. Plasmid encoding shRNA against CD105 under the control of the constitutive U6 promoter was used as a control. We demonstrated the antiproliferative and antiangiogenic effects of both plasmids in SVEC4-10 cells, as well as a moderate antitumor and pronounced antimetastatic effect in B16F10-luc tumors in vivo. Our results provide evidence that targeting melanoma with shRNA molecules against CD105 under the control of endothelin-1 promoter is a feasible and effective treatment, especially for the reduction of metastatic spread.
    Vir: Molecular therapy. Nucleic acids [Elektronski vir]. - ISSN 2162-2531 (No. 4, May 2015)
    Vrsta gradiva - e-članek
    Leto - 2015
    Jezik - angleški
    COBISS.SI-ID - 1537399748

    Povezava(-e):

    http://www.nature.com/mtna/journal/v4/n5/full/mtna201512a.html
    http://www.ncbi.nlm.nih.gov/pubmed/25942402
    Repozitorij Univerze na Primorskem - RUP
    DOI

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