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Detecting structural variants and associated gene presence–absence variation phenomena in the genomes of marine organismsSollitto, Marco ...As complete genomes become easier to attain, even from previously difficult-to-sequence species, and as genomic resequencing becomes more routine, it is becoming obvious that genomic structural ... variation is more widespread than originally thought and plays an important role in maintaining genetic variation in populations. Structural variants (SVs) and associated gene presence–absence variation (PAV) can be important players in local adaptation, allowing the maintenance of genetic variation and taking part in other evolutionarily relevant phenomena. While recent studies have highlighted the importance of structural variation in Mollusca, the prevalence of this phenomenon in the broader context of marine organisms remains to be fully investigated. Here, we describe a straightforward and broadly applicable method for the identification of SVs in fully assembled diploid genomes, leveraging the same reads used for assembly. We also explain a gene PAV analysis protocol, which could be broadly applied to any species with a fully sequenced reference genome available. Although the strength of these approaches have been tested and proven in marine invertebrates, which tend to have high levels of heterozygosity, possibly due to their lifestyle traits, they are also applicable to other species across the tree of life, providing a ready means to begin investigations into this potentially widespread phenomena.Vir: Marine genomics : methods and protocols (Str. 53-76)Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasleLeto - 2022Jezik - angleškiCOBISS.SI-ID - 154387459
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Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Sollitto, Marco | 57435 |
Kenny, Nathan J. | ![]() |
Greco, Samuele | ![]() |
Tucci, Carmen Federica | ![]() |
Calcino, Andrew D. | ![]() |
Gerdol, Marco | ![]() |
Vir: Osebne bibliografije
in: SICRIS
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