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  • Quantification and whole genome characterization of SARS-CoV-2 RNA in wastewater and air samples [Elektronski vir]
    Gonçalves, José ...
    Wastewater-based epidemiology has emerged as a promising and efficacious surveillance system for SARS-CoV-2 and other infectious diseases in many nations. The process typically involves wastewater ... concentration, nucleic acid extraction, amplification of selected genomic segments, and detection and quantification of the amplified genomic segment. This methodology can similarly be leveraged to detect and quantify infectious agents, such as SARS-CoV-2, in air samples. Initially, SARSCoV- 2 was presumed to spread primarily through close personal contact with droplets generated by an infected individual while speaking, sneezing, coughing, singing, or breathing. However, a growing number of studies have reported the presence of SARS-CoV-2 RNA in the air of healthcare facilities, establishing airborne transmission as a viable route for the virus. This study presents a composite of established protocols to facilitate environmental detection, quantification, and sequencing of viruses from both wastewater and air samples.
    Vir: Journal of visualized experiments [Elektronski vir]. - ISSN 1940-087X (Vol. 196, 2023, str. 1-15)
    Vrsta gradiva - e-članek ; neleposlovje za odrasle
    Leto - 2023
    Jezik - angleški
    COBISS.SI-ID - 157657859

    Povezava(-e):

    https://www.jove.com/t/65053/quantification-whole-genome-characterization-sars-cov-2-rna
    DOI

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