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  • A cka-gfp transcriptional fusion reveals that the Colicin K activity gene isinduced in only 3 percent of the population
    Mulec, Janez, 1975- ...
    In prokaryotes, oniy a few examples of differential gene expression in cell populations have been described. Colicin production in natura! populations of Escherichia coli, while providing a ... competitive advantage in the natural habitat, also leads to lysis of the toxin-producing cell. Colicin K synthesis has been found to be induced due to an increase in ppGpp (I. Kuhar, J. P. van Putten, D. Žgur-Bertok, W. Gaastra, and B. J. Jordi, Mol. Microbiol 41:207-216). Using two transcriptional fusions, cka-gfp and cki:gfp, we show that at the single-cell level, the colicin K activity gene cka is expressed inonly 3% of the bacterial population upon induction by nutrient starvation. In contrast, the immunity gene cki is expressed in the large majority of the cells. Expression of the cka-gfp fusion in a lexA-defective strain and in a relA spoT mutant strain indicates that differential expression of cka is established primarily at the level of transcription.
    Vir: Journal of bacteriology. - ISSN 0021-9193 (Letn. 185, št. 2, 2003, str. 654-659)
    Vrsta gradiva - članek, sestavni del
    Leto - 2003
    Jezik - angleški
    COBISS.SI-ID - 15824089

vir: Journal of bacteriology. - ISSN 0021-9193 (Letn. 185, št. 2, 2003, str. 654-659)
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