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Določanje celokupnega plazemskega homocisteina = Determination of total plasma homocysteineBožič, Mojca, 1970- ...Numerous studies performed in recent years have confirmed that elevated total plasma homocysteine (cHCE) presents an independent risk factor for vascular diseases such as peripheral artery occlusive ... disease, ischemic stroke, myocardial infarction and venous thrombosis. In order to detect subjects who are at a higher risk for vascular disease, increasing demands were placed on laboratories to measure cHCE, which has lead to development of numerous chromatographic and immunoassay methods. The aim of this study was to test different methods for cHCE determination and to asses, which of the methods are suitable for clinical laboratory. We tested two chromatographic methods (amino-acid analyser and high-pressure liquid chromatography with fluorimetricdetection) and two immunoassayćs (fluorescence immunoassay and enzyme-immuno assay). The characteristics of all tested methods were good. However, interassay variability and technical demands were higher for amino-acid analyser, therefore this method was excluded from further comparison. High-pressure liquid chromatography was in agreement with both immunoassays, whereas systemic bias was observed between the two immunoassays.We conclude, that high-pressure liquid chromatography with fluorimetric detection is suitable for use in a clinical laboratory. Fluorescence immunoassay could substitute this method in laboratories without chromatography equipment, while enzyme-linked immunoassay could be utilised inlaboratories with high sample throughput.Vir: Farmacevtski vestnik : strokovno glasilo slovenske farmacije = pharmaceutical journal of Slovenia. - ISSN 0014-8229 (Vol. 52, 2001, str. 19-24)Vrsta gradiva - članek, sestavni delLeto - 2001Jezik - slovenskiCOBISS.SI-ID - 15954983
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vir: Farmacevtski vestnik : strokovno glasilo slovenske farmacije = pharmaceutical journal of Slovenia. - ISSN 0014-8229 (Vol. 52, 2001, str. 19-24)
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Božič, Mojca, 1970- | 15637 |
Skitek, Milan | 11331 |
Ritonja, Anka | 05266 |
Jerin, Aleš | 17160 |
Stegnar, Mojca | 05034 |
Vir: Osebne bibliografije
in: SICRIS
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