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Human osteoblast-like osteosarcoma cell adhesion on nanosecond laser-textured 316L stainless steel surfaces [Elektronski vir]Hočevar, Matej, 1982- ...Although stainless steel is widely used in medicine due to good combination of mechanical properties durability, ease of fabrication and low cost, it often exhibit insufficient biocompatibility. The ... mechanical performance of material is governed by its bulk properties, while biocompatibility is determined by the properties of its surface. Therefore, considerable effort is being devoted to the surface engineering of biomaterials in order to improve the material - cell interactions. Laser surface processing enables modification on micro - and nanoscale that significantly change surface properties and influence cellular adhesion. Within this context, the re sponse of human osteoblast - like osteosarcoma cells (MG63) to laser textured 316L stainless steel was investigated. Surface texturing was carried out by a nanosecond laser at high fluences. Surface properties of non - treated and laser textured samples were a nalysed using scanning electron microscopy (SEM), X - ray photoelectron spectroscopy (XPS), optical 3D surface measuring system and contact angle goniometer was employed for surface wettability measurements. The influence of micro - and nanoscale transformati on on MG63 cell behaviour was assessed by in - vitro study using fluorescence (viable staining) and SEM microscopy after 24 h of exposure. Surface characterization revealed significant differences in surface morphology, chemistry and wettability after laser texturing. Non - treated sample exhibit hydrophobic grain - like structured surface that transforms to hierarchical micro - and nanostructured surface covered with thick oxides super - hydrophilic nature. After 24 hours of exposure, cells tend to attach in higher numbers to non - treated surface. On the other hand, we observed that cells respond to the laser - textured surfaces. Cells tend to attach and preferentially align on the surface between laser - induced micro - grooves oriented in a specific direction. Furthermor e, nanostructured oxide surface also induces changes in surface morphology of cells while no cytotoxic effects were observed. Nd:YAG nanosecond laser surface texturing presents flexible, simple and chemical free approach that can be applied to most metalli c materials without affecting the properties of bulk material.Vrsta gradiva - prispevek na konferenciLeto - 2018Jezik - angleškiCOBISS.SI-ID - 16285723
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Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Hočevar, Matej, 1982- | 32545 |
Gregorčič, Peter, fizik | 29224 |
Šetina, Barbara | 25498 |
Kononenko, Veno | 35369 |
Drobne, Damjana | 11155 |
Godec, Matjaž, 1961- | 10842 |
Vir: Osebne bibliografije
in: SICRIS
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