VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • A model of repression: CTD analogs and PIE-1 inhibit transcriptional elongation by P-TEFb
    Zhang, Fan, medicina ...
    The positive transcription elongation factor b (P-TEFb) contains cyclin T1 (CycT1) and cyclin-dependent kinase 9 (Cdk9). For activating the expression ofeukaryotic genes, the histidine-rich sequence ... in CycT1 binds the heptapeptide repeats in the C-terminal domain (CTD) of RNA polymerase II (RNAPII), whereupon Cdk9 phosphorylates the CTD. We found that alanine-substituted heptapeptide repeats that cannot be phosphorylated also bind CycT1. When placed near transcription units, these CTD analogs block effects of P-TEFb. Remarkably, the transcriptional repressor PIE-1 from Caenorhabditis elegans behaves analogously. It binds CycT1 via an alanine-containing heptapeptide repeat and inhibits transcriptional elongation. Thus, our findings reveal a new mechanism by which repressors inhibit eukaryotic transcription.
    Vir: Genes & development. - ISSN 0890-9369 (Letn. 17, št. 6, 2003, str. 748-758)
    Vrsta gradiva - članek, sestavni del
    Leto - 2003
    Jezik - angleški
    COBISS.SI-ID - 16469465

vir: Genes & development. - ISSN 0890-9369 (Letn. 17, št. 6, 2003, str. 748-758)
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