VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Detection of specific antibodies against SARS-CoV-2 spike protein via ultra-sensitive bio-functionalized carbonnitride-reduced graphene oxide electrochemical immunosensing platform in real samples [Elektronski vir]
    Abubakar Sadique, Mohd. ...
    In this work, we fabricate a facile de novo synthesis route to prepare bio-functionalized carbon nitride-reduced graphene oxide with chitosan (Chi) nanocomposite (C3N4/RGO/Chi). The nitrogen-rich ... C3N4 has been synthesized through facile and low-cost thermal oxidation and offers a metal-free and highly active surface for synergistic modification with RGO and Chi. Moreover, the synergistically incorporated C3N4/RGO and biopolymer improve the electrocatalytic activity, provide surface functionalities, and enhance electroconductivity for immunosensing applications. Consequently, the bio-functionalized C3N4/RGO nanocomposite provides a suitable substrate for the immobilization of SARS-CoV-2 RBD spike protein. The immunosensor detects SARS-CoV-2 antibodies in a wide detection range from 10 zg mL−1 (10 × 10−21 g mL−1) to 100 ng mL−1 with an ultra-low detection limit (LOD) of 3.31 zg mL−1. The validation of the immunosensor was also carried out on spiked serum samples, which show a linear detection range of 100 ag mL−1 to 100 ng mL−1 and a LOD of 1.73 ag mL−1. Nevertheless, the clinical diagnostic application of the immunosensor is validated through the examination of real serum samples from COVID-19 patients. The results suggest that the immunosensor can be efficiently used as a screening platform to distinguish between positive and negative samples with high accuracy. The findings of the work have the potential to translate the immunosensing strategy into next-generation point-of-care testing (POCT) of various infectious diseases.
    Vir: Materials advances [Elektronski vir]. - ISSN 2633-5409 (iss. 21, Vol. 4, Nov. 2023, str. 5291-5304)
    Vrsta gradiva - e-članek ; neleposlovje za odrasle
    Leto - 2023
    Jezik - angleški
    COBISS.SI-ID - 168038659

    Povezava(-e):

    https://pubs.rsc.org/en/content/articlelanding/2023/ma/d3ma00399j
    DOI

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