VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Fast and accurate multiplex... (naslovnica)
    Fast and accurate multiplex identification and quantification of seven genetically modified soybean lines using six-color digital PCR [Elektronski vir]
    Bogožalec Košir, Alexandra ...
    The proliferation of genetically modified organisms (GMOs) presents challenges to GMO testing laboratories and policymakers. Traditional methods, like quantitative real-time PCR (qPCR), face ... limitations in quantifying the increasing number of GMOs in a single sample. Digital PCR (dPCR), specifically multiplexing, offers a solution by enabling simultaneous quantification of multiple GMO targets. This study explores the use of the Naica six-color Crystal dPCR platform for quantifying five GM soybean lines within a single six-plex assay. Two four-color assays were also developed for added flexibility. These assays demonstrated high specificity, sensitivity (limit of detection or LOD < 25 copies per reaction) and precision (bias to an estimated copy number concentration <15%). Additionally, two approaches for the optimization of data analysis were implemented. By applying a limit-of-blank (LOB) correction, the limit of quantification (LOQ) and LOD could be more precisely determined. Pooling of reactions additionally lowered the LOD, with a two- to eight-fold increase in sensitivity. Real-life samples from routine testing were used to confirm the assays’ applicability for quantifying GM soybean lines in complex samples. This study showcases the potential of the six-color Crystal dPCR platform to revolutionize GMO testing, facilitating comprehensive analysis of GMOs in complex samples.
    Vir: Foods [Elektronski vir]. - ISSN 2304-8158 (Vol. 12, iss. 22, art. no. 4156, Nov. 2023, str. [1]-17)
    Vrsta gradiva - e-članek
    Leto - 2023
    Jezik - angleški
    COBISS.SI-ID - 173360131

    Povezava(-e):

    https://doi.org/10.3390/foods12224156
    DiRROS - Digitalni repozitorij raziskovalnih organizacij Slovenije
    DOI

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