VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • A label-free light-scattering method to resolve assembly and disassembly of DNA nanostructures [Elektronski vir]
    Ijäs, Heini ...
    DNA self-assembly, and in particular DNA origami, has evolved into a reliable workhorse for organizing organic and inorganic materials with nanometer precision and with exactly controlled ... stoichiometry. To ensure the intended performance of a given DNA structure, it is beneficial to determine its folding temperature, which in turn yields the best possible assembly of all DNA strands. Here, we show that temperature-controlled sample holders and standard fluorescence spectrometers or dynamic light-scattering setups in a static light-scattering configuration allow for monitoring the assembly progress in real time. With this robust label-free technique, we determine the folding and melting temperatures of a set of different DNA origami structures without the need for more tedious protocols. In addition, we use the method to follow digestion of DNA structures in the presence of DNase I and find strikingly different resistances toward enzymatic degradation depending on the structural design of the DNA object.
    Vir: Biophysical journal [Elektronski vir]. - ISSN 1542-0086 (Vol. 121, iss. 24, Dec. 2022, Str. 4800-4809)
    Vrsta gradiva - e-članek ; neleposlovje za odrasle
    Leto - 2022
    Jezik - angleški
    COBISS.SI-ID - 175138819

    Povezava(-e):

    https://www.sciencedirect.com/science/article/pii/S0006349522008748
    DOI

Vnos na polico